Hotspot

clpP-psbB

LSC · 73850-74306 · 457 bp

Back to Platymiscium

Pi 0.122

MarkerSeek score 87.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 457
variable_sites: 53
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CAAGATGCATCTTCCTCCCC	AAACGGCCGGGATCATTTAA	55.0	45.0	58.0	57.9	566-572 bp	1.0	89.5
clpP-psbB_p2	CAAGATGCATCTTCCTCCCC	TGGACAGAAAGTAAACGGCC	55.0	50.0	58.0	58.1	578-584 bp	1.0	89.4
clpP-psbB_p3	CCAAGATGCATCTTCCTCCC	TGGACAGAAAGTAAACGGCC	55.0	50.0	58.0	58.1	579-585 bp	1.0	89.4
clpP-psbB_p4	CCCAAGATGCATCTTCCTCC	TGGACAGAAAGTAAACGGCC	55.0	50.0	58.0	58.1	580-586 bp	1.0	89.4
clpP-psbB_p5	CCAAGATGCATCTTCCTCCC	AAACGGCCGGGATCATTTAA	55.0	45.0	58.0	57.9	567-573 bp	1.0	89.4