Hotspot

psbE-petL

LSC · 65882-66911 · 1030 bp

Back to Platymiscium

Pi 0.0462

MarkerSeek score 79.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1030
variable_sites: 46
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAGCAAAAGAACGTTCTCCG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.7	58.0	1105-1126 bp	1.0	83.4
psbE-petL_p2	CAGCAAAAGAACGTTCTCCG	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.7	58.0	1107-1128 bp	1.0	83.3
psbE-petL_p3	CAAAAGAACGTTCTCCGGTG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.3	58.0	1102-1123 bp	1.0	80.5
psbE-petL_p4	CAAAAGAACGTTCTCCGGTG	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.3	58.0	1104-1125 bp	1.0	80.5
psbE-petL_p5	AGCAAAAGAACGTTCTCCGG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.8	58.0	1104-1125 bp	1.0	79.6