Hotspot

petA-psbJ

LSC · 63800-65103 · 1304 bp

Back to Platymiscium

Pi 0.0385

MarkerSeek score 81.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1304
variable_sites: 47
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGAAGGTGCAATTGTCCGAG	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1329-1365 bp	1.0	80.8
petA-psbJ_p2	GAAGGTGCAATTGTCCGAGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1328-1364 bp	1.0	80.7
petA-psbJ_p3	GAGAAGGTGCAATTGTCCGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1330-1366 bp	1.0	80.7
petA-psbJ_p4	AGGTGCAATTGTCCGAGATG	CATATTCCGGATTGGGCTCA	50.0	50.0	58.3	57.4	1326-1362 bp	1.0	80.2
petA-psbJ_p5	GGTGCAATTGTCCGAGATGA	CATATTCCGGATTGGGCTCA	50.0	50.0	58.3	57.4	1325-1361 bp	1.0	80.2