Hotspot

trnQ-UUG-rps16

LSC · 56518-57041 · 524 bp

Back to Platymiscium

Pi 0.0594

MarkerSeek score 81.90

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 30
pi: 0.0594
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAGGTTCGAATCCTTCCGTC	GCACGTTGCTTTTTACCACA	55.0	45.0	58.1	57.8	590-592 bp	1.0	84.5
trnQ-UUG-rps16_p2	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	604 bp	0.5	59.3
trnQ-UUG-rps16_p3	GAGGTTCGAATCCTTCCGTC	ATGTCTTTCAAGTCGCACGT	55.0	45.0	58.1	58.1	606 bp	0.5	58.3
trnQ-UUG-rps16_p4	GAGGTTCGAATCCTTCCGTC	CATGTCTTTCAAGTCGCACG	55.0	50.0	58.1	57.8	607 bp	0.5	57.7
trnQ-UUG-rps16_p5	GAGGTTCGAATCCTTCCGTC	TGTCTTTCAAGTCGCACGTT	55.0	45.0	58.1	58.4	605 bp	0.5	56.6