Hotspot

psbK-trnQ-UUG

LSC · 55887-56445 · 559 bp

Back to Platymiscium

Pi 0.0919

MarkerSeek score 84.10

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 50
pi: 0.0919
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	ACTTGAATAAAGGGCAGAGTGA	GATATGCTCTGGGACGGAAG	40.9	55.0	57.4	57.6	696-702 bp	1.0	62.7
psbK-trnQ-UUG_p2	ACTTGAATAAAGGGCAGAGTGA	GACCAAAACCCGTTGCCTTA	40.9	50.0	57.4	58.7	654-660 bp	1.0	61.4
psbK-trnQ-UUG_p3	ACTTGAATAAAGGGCAGAGTGAA	GATATGCTCTGGGACGGAAG	39.1	55.0	58.0	57.6	696-702 bp	1.0	59.7
psbK-trnQ-UUG_p4	ACTTGAATAAAGGGCAGAGTGA	AATGGATATGCTCTGGGACG	40.9	50.0	57.4	57.2	700-706 bp	1.0	59.5
psbK-trnQ-UUG_p5	ACTTGAATAAAGGGCAGAGTGA	GAATAGCGGGACCAAAACCC	40.9	55.0	57.4	58.9	663-669 bp	1.0	59.5