Hotspot

trnG-UCC-trnS-GCU

LSC · 54178-54980 · 803 bp

Back to Platymiscium

Pi 0.0714
MarkerSeek score 81.40
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
803
variable_sites
46
pi
0.0714
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 GGAGTCCGTAGAACGAATCG CAATCCGACGCTTTAGTCCA 55.0 50.0 57.9 58.0 743-884 bp 1.0 87.7
trnG-UCC-trnS-GCU_p2 GGAGTCCGTAGAACGAATCG AATCCGACGCTTTAGTCCAC 55.0 50.0 57.9 58.0 742-883 bp 1.0 87.7
trnG-UCC-trnS-GCU_p3 GGAGTCCGTAGAACGAATCG ACGGAAAGAGAGGGATTCGA 55.0 50.0 57.9 58.2 796-937 bp 1.0 86.2
trnG-UCC-trnS-GCU_p4 CCGTAGAACGAATCGCACTT CAATCCGACGCTTTAGTCCA 50.0 50.0 58.4 58.0 738-879 bp 1.0 85.5
trnG-UCC-trnS-GCU_p5 CCGTAGAACGAATCGCACTT AATCCGACGCTTTAGTCCAC 50.0 50.0 58.4 58.0 737-878 bp 1.0 85.5