Hotspot

trnT-GGU-trnE-UUC

LSC · 30399-31093 · 695 bp

Back to Platymiscium

Pi 0.0407

MarkerSeek score 78.60

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 25
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	AGAGAGATGTCCTGGACCAC	50.0	55.0	58.0	58.1	722-784 bp	1.0	85.5
trnT-GGU-trnE-UUC_p2	GAACCGATGACTTACGCCTT	GAGAGATGTCCTGGACCACT	50.0	55.0	58.0	58.1	721-783 bp	1.0	85.5
trnT-GGU-trnE-UUC_p3	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	58.0	57.8	737-799 bp	1.0	85.2
trnT-GGU-trnE-UUC_p4	GGTACAAAGCCCCTTATCGG	AGAGAGATGTCCTGGACCAC	55.0	55.0	58.0	58.1	746-808 bp	1.0	85.2
trnT-GGU-trnE-UUC_p5	GGTACAAAGCCCCTTATCGG	GAGAGATGTCCTGGACCACT	55.0	55.0	58.0	58.1	745-807 bp	1.0	85.2