Hotspot

trnG-GCC-psbZ

LSC · 25402-26136 · 735 bp

Back to Platymiscium

Pi 0.0801

MarkerSeek score 81.20

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 56
pi: 0.0801
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTTGTTTTTCGACCGGAAG	CTTCTCCTGATGGTTGGTCG	50.0	55.0	58.0	58.0	1381-1385 bp	1.0	86.2
trnG-GCC-psbZ_p2	CTTGAGGTCACGGGTTCAAA	TTTCGGGCTCGGCTATACTA	50.0	50.0	58.0	58.0	1674-1675 bp	1.0	85.6
trnG-GCC-psbZ_p3	CGTTGTTTTTCGACCGGAAG	CAACCACTCAGCCATCTCTC	50.0	55.0	58.0	58.0	1827-1834 bp	1.0	85.4
trnG-GCC-psbZ_p4	CGTTGTTTTTCGACCGGAAG	TTTCGGGCTCGGCTATACTA	50.0	50.0	58.0	58.0	2005-2012 bp	1.0	85.2
trnG-GCC-psbZ_p5	CGTTGTTTTTCGACCGGAAG	TACTACCTCTTAGCCGAGCC	50.0	55.0	58.0	58.0	1990-1997 bp	1.0	85.2