Hotspot

ndhJ

LSC · 11566-12042 · 477 bp

Back to Platylobium

Pi 0.00992

MarkerSeek score 38.00

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: LSC
length_bp: 477
variable_sites: 10
pi: 0.00992
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	AGTATCCTCATCCTCCCACG	CCTCGTGTCACCAGTTCAAA	55.0	50.0	58.0	58.1	1298-1308 bp	1.0	82.5
ndhJ_p2	GTATCCTCATCCTCCCACGA	CCTCGTGTCACCAGTTCAAA	55.0	50.0	58.0	58.1	1297-1307 bp	1.0	82.5
ndhJ_p3	AGTATCCTCATCCTCCCACG	AGAATGGTCGGGATAGCTCA	55.0	50.0	58.0	57.9	1343-1353 bp	1.0	82.1
ndhJ_p4	GTATCCTCATCCTCCCACGA	AGAATGGTCGGGATAGCTCA	55.0	50.0	58.0	57.9	1342-1352 bp	1.0	82.1
ndhJ_p5	TATAGTACAGTTCGGGGCGT	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.9	58.1	1594-1609 bp	1.0	82.0