Hotspot

rps15-ycf1

SSC · 120202-120548 · 347 bp

Back to Platylobium

Pi 0.0148

MarkerSeek score 48.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 347
variable_sites: 11
pi: 0.0148
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	AGCGCTTCCGTTGATTCATA	45.0	45.0	58.1	57.7	80-1324 bp	1.0	80.4
rps15-ycf1_p2	ATTTCCCAACTGTTCCGGTC	AGCGCTTCCGTTGATTCATA	50.0	45.0	58.1	57.7	80-1522 bp	1.0	79.8
rps15-ycf1_p3	CCCAGCCATAGTAGGTGAGA	AGCGCTTCCGTTGATTCATA	55.0	45.0	57.9	57.7	80-1605 bp	1.0	79.7
rps15-ycf1_p4	TCCCAGCCATAGTAGGTGAG	AGCGCTTCCGTTGATTCATA	55.0	45.0	57.9	57.7	80-1606 bp	1.0	79.7
rps15-ycf1_p5	TCATTGTTCCACGAATCGCT	AGCGCTTCCGTTGATTCATA	45.0	45.0	57.9	57.7	80-1234 bp	0.666667	66.9