Hotspot

ndhG-ndhI

SSC · 115259-115710 · 452 bp

Back to Platylobium

Pi 0.0167

MarkerSeek score 57.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 452
variable_sites: 14
pi: 0.0167
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTGCTGCAGCTACAAAATGG	TGGTCAACAAACAGTCCGAG	50.0	50.0	58.0	58.1	80-1090 bp	1.0	83.6
ndhG-ndhI_p2	TCTGTGCTGCAGCTACAAAA	TGGTCAACAAACAGTCCGAG	45.0	50.0	58.0	58.1	80-1093 bp	1.0	83.4
ndhG-ndhI_p3	ATCTGTGCTGCAGCTACAAA	TGGTCAACAAACAGTCCGAG	45.0	50.0	57.8	58.1	80-1094 bp	1.0	82.2
ndhG-ndhI_p4	GTGCTGCAGCTACAAAATGG	GTCCGAGCTGCAAGATACAT	50.0	50.0	58.0	57.8	80-1077 bp	1.0	82.2
ndhG-ndhI_p5	TTGCACCTATCAAAGCAGCT	TGGTCAACAAACAGTCCGAG	45.0	50.0	57.8	58.1	80-1417 bp	1.0	81.3