Hotspot

rpl32-trnL-UAG

SSC · 110042-110626 · 585 bp

Back to Platylobium

Pi 0.017
MarkerSeek score 40.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
585
variable_sites
19
pi
0.017
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAGGGGATATTGGACAGCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.8 57.9 80-749 bp 1.0 81.6
rpl32-trnL-UAG_p2 TGAGATAGGAAGGGGGTTCG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.2 57.9 80-839 bp 1.0 81.1
rpl32-trnL-UAG_p3 AGAGGGGATATTGGACAGCA AGCAGCGTGTCTACCAATTT 50.0 45.0 57.8 57.8 80-742 bp 1.0 81.1
rpl32-trnL-UAG_p4 AGAGGGGATATTGGACAGCA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.8 57.8 80-748 bp 1.0 81.0
rpl32-trnL-UAG_p5 AGAGGGGATATTGGACAGCA CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.8 57.8 80-747 bp 1.0 81.0