Hotspot

psaJ-rpl33

LSC · 64326-64872 · 547 bp

Back to Platylobium

Pi 0.0194

MarkerSeek score 54.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 21
pi: 0.0194
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TACTCTATGGTTCGGGGCTT	CCTTTACCCTTCTTGGCCAT	50.0	50.0	57.8	57.5	80-672 bp	1.0	77.6
psaJ-rpl33_p2	GCTTTAGCGGGTCTCTTGAT	CCTTTACCCTTCTTGGCCAT	50.0	50.0	57.7	57.5	80-656 bp	1.0	76.5
psaJ-rpl33_p3	CTATGGTTCGGGGCTTTAGC	CCTTTACCCTTCTTGGCCAT	55.0	50.0	58.4	57.5	80-668 bp	1.0	75.8
psaJ-rpl33_p4	TACTCTATGGTTCGGGGCTT	ACCTTTACCCTTCTTGGCCA	50.0	50.0	57.8	58.8	80-673 bp	1.0	75.1
psaJ-rpl33_p5	GTACTCTATGGTTCGGGGCT	CCTTTACCCTTCTTGGCCAT	55.0	50.0	58.6	57.5	80-673 bp	1.0	74.3