Hotspot

trnP-UGG-psaJ

LSC · 63519-64190 · 672 bp

Back to Platylobium

Pi 0.0101

MarkerSeek score 57.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 14
pi: 0.0101
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TACCAAACTGCGCTACATCC	CGAGAGATATGTTTTTAGATCTCGC	50.0	40.0	58.0	57.7	80-721 bp	1.0	45.9
trnP-UGG-psaJ_p2	AAACAAACGCGCTACCAAAC	CGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	58.2	57.7	80-733 bp	1.0	45.8
trnP-UGG-psaJ_p3	CAAAACAAACGCGCTACCAA	CGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	58.2	57.7	80-735 bp	1.0	45.8
trnP-UGG-psaJ_p4	TTGTACCCAAAACAAACGCG	CGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	57.8	57.7	80-742 bp	1.0	45.8
trnP-UGG-psaJ_p5	TACCCAAAACAAACGCGCTA	CGAGAGATATGTTTTTAGATCTCGC	45.0	40.0	58.4	57.7	80-739 bp	1.0	45.8