Hotspot

trnW-CCA-trnP-UGG

LSC · 63276-63444 · 169 bp

Back to Platylobium

Pi 0.0398

MarkerSeek score 66.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 13
pi: 0.0398
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GGATGTAGCGCAGTTTGGTA	50.0	50.0	57.9	58.0	80-269 bp	1.0	87.8
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	GGATGTAGCGCAGTTTGGTA	50.0	50.0	57.9	58.0	80-296 bp	1.0	87.0
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	GGAAACCAACGGAAAGGGAT	50.0	50.0	57.9	58.1	80-285 bp	1.0	87.0
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	GGAAACCAACGGAAAGGGAT	50.0	50.0	57.9	58.1	80-312 bp	1.0	86.1
trnW-CCA-trnP-UGG_p5	TGGAATCACGCTCTGTAGGA	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.2	58.0	80-321 bp	1.0	85.9