Hotspot

rps16-accD

LSC · 55355-55808 · 454 bp

Back to Platylobium

Pi 0.0148

MarkerSeek score 45.70

Primer available yes

Label rps16-accD

Feature Metadata

label_name: rps16-accD
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 13
pi: 0.0148
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-accD_p1	CGTACGAGGCGAAAACTTCT	GAAATTCTGCATGTTCCGGC	50.0	50.0	58.3	58.1	80-986 bp	1.0	82.4
rps16-accD_p2	GCAATTGATGTTCGATCCCG	GAAATTCTGCATGTTCCGGC	50.0	50.0	57.6	58.1	80-893 bp	1.0	81.2
rps16-accD_p3	CGTACGAGGCGAAAACTTCT	CCGGCTGACATTGACTGAAT	50.0	50.0	58.3	58.3	80-971 bp	1.0	81.1
rps16-accD_p4	GCCGTTTATCGAATCGTTGC	GAAATTCTGCATGTTCCGGC	50.0	50.0	58.3	58.1	80-911 bp	0.666667	65.2
rps16-accD_p5	GCCGTTTATCGAATCGTTGC	CCGGCTGACATTGACTGAAT	50.0	50.0	58.3	58.3	80-896 bp	0.666667	63.8