Hotspot

psbK-rps16

LSC · 53150-54162 · 1013 bp

Back to Platylobium

Pi 0.0137

MarkerSeek score 58.00

Primer available yes

Label psbK-rps16

Feature Metadata

label_name: psbK-rps16
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 28
pi: 0.0137
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-rps16_p1	AGCAGCTTGCCAAACAAAAG	TGGCAGCAACATACCCTTTT	45.0	45.0	58.1	58.0	80-1650 bp	1.0	84.0
psbK-rps16_p2	CTTACAGCAGCTTGCCAAAC	TGGCAGCAACATACCCTTTT	50.0	45.0	57.9	58.0	80-1655 bp	1.0	83.9
psbK-rps16_p3	AGGCTTCGGGTAATTTCGTC	TGGCAGCAACATACCCTTTT	50.0	45.0	58.0	58.0	80-1567 bp	0.666667	67.1
psbK-rps16_p4	AGGCTTCGGGTAATTTCGTC	TCAACTCGAGCTCCATTGTG	50.0	50.0	58.0	57.9	80-1330 bp	0.666667	66.5
psbK-rps16_p5	AGGCTTCGGGTAATTTCGTC	CCCCCAACCCCATTCTAGTA	50.0	55.0	58.0	58.1	80-1269 bp	0.666667	66.5