Hotspot

ycf3-psaA

LSC · 17856-18742 · 887 bp

Back to Platylobium

Pi 0.0109

MarkerSeek score 61.00

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 887
variable_sites: 19
pi: 0.0109
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AAGATCACCGAGCGTTTTGA	TTTGACTTCTGGTTCCGGTG	45.0	50.0	57.8	58.0	896-944 bp	1.0	84.0
ycf3-psaA_p2	AGATCACCGAGCGTTTTGAA	TTTGACTTCTGGTTCCGGTG	45.0	50.0	57.8	58.0	895-943 bp	1.0	84.0
ycf3-psaA_p3	TGAAGATCACCGAGCGTTTT	TTTGACTTCTGGTTCCGGTG	45.0	50.0	57.8	58.0	898-946 bp	1.0	84.0
ycf3-psaA_p4	TTGAAGATCACCGAGCGTTT	TTTGACTTCTGGTTCCGGTG	45.0	50.0	57.8	58.0	899-947 bp	1.0	84.0
ycf3-psaA_p5	TTGGTTGAAGATCACCGAGC	TTTGACTTCTGGTTCCGGTG	50.0	50.0	58.2	58.0	903-951 bp	1.0	83.8