Hotspot

trnL-UAA-trnT-UGU

LSC · 13443-14284 · 842 bp

Back to Platylobium

Pi 0.0165

MarkerSeek score 63.40

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 30
pi: 0.0165
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.1	57.9	587-900 bp	1.0	83.1
trnL-UAA-trnT-UGU_p2	CCGTACCAAGGCTCAATTCA	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.9	57.9	617-930 bp	1.0	82.6
trnL-UAA-trnT-UGU_p3	GTTTCCGTACCAAGGCTCAA	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.1	57.9	621-934 bp	1.0	82.6
trnL-UAA-trnT-UGU_p4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	50.0	50.0	58.1	57.8	586-932 bp	1.0	82.1
trnL-UAA-trnT-UGU_p5	AAGTTTCCGTACCAAGGCTC	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	623-936 bp	1.0	82.0