Hotspot

ndhJ-trnF-GAA

LSC · 12043-12693 · 651 bp

Back to Platylobium

Pi 0.0135
MarkerSeek score 58.10
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
LSC
length_bp
651
variable_sites
18
pi
0.0135
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 GGCTGGCCTTTACGTAAAGA CCTCGTGTCACCAGTTCAAA 50.0 50.0 57.9 58.1 738-748 bp 1.0 82.3
ndhJ-trnF-GAA_p2 AGGCTGGCCTTTACGTAAAG CCTCGTGTCACCAGTTCAAA 50.0 50.0 57.9 58.1 739-749 bp 1.0 82.2
ndhJ-trnF-GAA_p3 GGCTGGCCTTTACGTAAAGA AGAATGGTCGGGATAGCTCA 50.0 50.0 57.9 57.9 783-793 bp 1.0 81.8
ndhJ-trnF-GAA_p4 AGGCTGGCCTTTACGTAAAG AGAATGGTCGGGATAGCTCA 50.0 50.0 57.9 57.9 784-794 bp 1.0 81.8
ndhJ-trnF-GAA_p5 CCGAAAGTTGGATAGGCTGG CCTCGTGTCACCAGTTCAAA 55.0 50.0 58.3 58.1 752-762 bp 1.0 80.4