Hotspot

trnP-GGG-trnL-UAG

SSC · 113454-113704 · 251 bp

Back to Platycerium

Pi 0.163

MarkerSeek score 82.90

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 39
pi: 0.163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	CGCTACCAAACTGTGCCATA	GGATTCGAACCGAGATGCTT	50.0	50.0	58.3	58.1	338-346 bp	1.0	88.0
trnP-GGG-trnL-UAG_p2	TGAACCTGTGACCTCCATCA	GGATTCGAACCGAGATGCTT	50.0	50.0	58.3	58.1	371-379 bp	1.0	87.7
trnP-GGG-trnL-UAG_p3	CGCTACCAAACTGTGCCATA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	308-316 bp	1.0	87.7
trnP-GGG-trnL-UAG_p4	CGCTACCAAACTGTGCCATA	CAGCGTGTCTACCTGTTTCA	50.0	50.0	58.3	57.9	299-307 bp	1.0	87.6
trnP-GGG-trnL-UAG_p5	TGAACCTGTGACCTCCATCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	341-349 bp	1.0	87.4