Hotspot

rrn16-rps12

IRb · 91680-98425 · 6746 bp

Back to Platycerium

Pi 0.354

MarkerSeek score 67.40

Primer available yes

Label rrn16-rps12

Feature Metadata

label_name: rrn16-rps12
feature_type: igs
region: IRb
length_bp: 6746
variable_sites: 1375
pi: 0.354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.58
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps12_p1	TCGTTGCCCGGACTATATCT	AAAGGAGAATGAGCTCGCAG	50.0	50.0	58.0	58.0	895 bp	0.5	59.8
rrn16-rps12_p2	TTCTAGCGCCCAGGATATGA	AAAGGAGAATGAGCTCGCAG	50.0	50.0	58.0	58.0	767 bp	0.5	59.8
rrn16-rps12_p3	CACACTGTGACCTATTCGGG	AAAGGAGAATGAGCTCGCAG	55.0	50.0	58.0	58.0	803 bp	0.5	59.7
rrn16-rps12_p4	CCACACTGTGACCTATTCGG	AAAGGAGAATGAGCTCGCAG	55.0	50.0	58.0	58.0	804 bp	0.5	59.7
rrn16-rps12_p5	TCGTTGCCCGGACTATATCT	GGATCTACCTGCTCCCAGAT	50.0	55.0	58.0	58.0	1036 bp	0.5	59.7