Hotspot

petA-psbJ

LSC · 57571-58251 · 681 bp

Back to Platycerium

Pi 0.123
MarkerSeek score 85.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
681
variable_sites
79
pi
0.123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCAGCTAGCAGAAATGAATT GTTGGGGTCGTCTCTTCAAT 40.0 50.0 54.3 57.5 713-728 bp 1.0 58.9
petA-psbJ_p2 GCAGCTAGCAGAAATGAATTT GTTGGGGTCGTCTCTTCAAT 38.1 50.0 55.2 57.5 713-728 bp 1.0 57.6
petA-psbJ_p3 GCAGCTAGCAGAAATGAATTTT GTTGGGGTCGTCTCTTCAAT 36.4 50.0 55.9 57.5 713-728 bp 1.0 55.7
petA-psbJ_p4 GCAGCTAGCAGAAATGAATTTTTG GTTGGGGTCGTCTCTTCAAT 37.5 50.0 57.9 57.5 713-728 bp 1.0 55.5
petA-psbJ_p5 GCAGCTAGCAGAAATGAATTTTT GTTGGGGTCGTCTCTTCAAT 34.8 50.0 56.6 57.5 713-728 bp 1.0 53.3