Hotspot

psbD-trnT-GGU

LSC · 31987-33346 · 1360 bp

Back to Platycerium

Pi 0.088

MarkerSeek score 85.50

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1360
variable_sites: 116
pi: 0.088
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TTTTCCGATGGCAATGGTCA	AACTCAGGGGTAGAGTGACG	45.0	55.0	58.1	58.5	1427-1445 bp	1.0	84.5
psbD-trnT-GGU_p2	ACGGTCTCTTCTTAGCCAGT	AACTCAGGGGTAGAGTGACG	50.0	55.0	58.1	58.5	1487-1505 bp	1.0	84.2
psbD-trnT-GGU_p3	TTTCCGATGGCAATGGTCAT	AACTCAGGGGTAGAGTGACG	45.0	55.0	57.9	58.5	1426-1444 bp	1.0	84.0
psbD-trnT-GGU_p4	CAACCCACGAAGACAAAACG	AACTCAGGGGTAGAGTGACG	50.0	55.0	57.9	58.5	1504-1522 bp	1.0	83.9
psbD-trnT-GGU_p5	ACCAACCCACGAAGACAAAA	AACTCAGGGGTAGAGTGACG	45.0	55.0	57.9	58.5	1506-1524 bp	1.0	83.7