Hotspot

rpoB-trnD-GUC

LSC · 24626-25269 · 644 bp

Back to Platycerium

Pi 0.0778

MarkerSeek score 80.80

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 48
pi: 0.0778
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	AATGTCAGCCTAACGTGTCC	55.0	50.0	58.0	57.9	1217 bp	0.5	59.2
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGG	AATGTCAGCCTAACGTGTCC	55.0	50.0	58.0	57.9	1218 bp	0.5	59.2
rpoB-trnD-GUC_p3	CCCCTTCGAATTCATCCAGG	AATGTCAGCCTAACGTGTCC	55.0	50.0	58.0	57.9	1504 bp	0.5	59.0
rpoB-trnD-GUC_p4	CCCTTCGAATTCATCCAGGG	AATGTCAGCCTAACGTGTCC	55.0	50.0	58.0	57.9	1503 bp	0.5	59.0
rpoB-trnD-GUC_p5	GTAGCATCCTCCGACGATTC	AATGTCAGCCTAACGTGTCC	55.0	50.0	57.9	57.9	1836 bp	0.5	58.8