Hotspot

atpH-atpI

LSC · 11795-12473 · 679 bp

Back to Platycerium

Pi 0.0905

MarkerSeek score 80.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 60
pi: 0.0905
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TCCCTCCTGGAAAGTTTTGC	AGGTGCCAGAAATCTGCAAA	50.0	45.0	58.0	58.0	1827-1831 bp	1.0	85.3
atpH-atpI_p2	TCCCTCCTGGAAAGTTTTGC	GAATACCGTTCCTGGGTTCC	50.0	55.0	58.0	58.0	1724-1728 bp	1.0	85.3
atpH-atpI_p3	GAGGGAGTAGCGATCTCACT	AGGTGCCAGAAATCTGCAAA	55.0	45.0	58.0	58.0	1866-1870 bp	1.0	85.0
atpH-atpI_p4	TCCCTCCTGGAAAGTTTTGC	TGGCAAGTAGGAGTCTTCCA	50.0	50.0	58.0	58.0	1913-1917 bp	1.0	85.0
atpH-atpI_p5	TCCCTCCTGGAAAGTTTTGC	TGGGGGAAGAAGAATACCGT	50.0	50.0	58.0	58.0	1735 bp	0.5	59.7