Hotspot

psbK-psbI

LSC · 5523-5944 · 422 bp

Back to Platycerium

Pi 0.126

MarkerSeek score 80.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 422
variable_sites: 48
pi: 0.126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCTGCAGTTAGCTTTCGCTA	ACAAATAGTTTGAGGGTAAGCA	50.0	36.4	58.6	55.6	429-466 bp	1.0	52.0
psbK-psbI_p2	GCTGCAGTTAGCTTTCGCTA	ACAAATAGTTTGAGGGTAAGCAT	50.0	34.8	58.6	56.2	429-466 bp	1.0	52.0
psbK-psbI_p3	TCTCCTAGCTTTTGTTTGGCA	ACAAATAGTTTGAGGGTAAGCA	42.9	36.4	57.8	55.6	451-488 bp	1.0	52.0
psbK-psbI_p4	GCTGCAGTTAGCTTTCGCTA	CAAATAGTTTGAGGGTAAGCA	50.0	38.1	58.6	53.8	428-465 bp	1.0	52.0
psbK-psbI_p5	GCTGCAGTTAGCTTTCGCTA	ACAAATAGTTTGAGGGTAAGC	50.0	38.1	58.6	53.8	429-466 bp	1.0	52.0