Hotspot

trnV-GAC-rps12

IRa · 142531-144214 · 1684 bp

Back to Platycarya

Pi 0.00139
MarkerSeek score 64.60
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1684
variable_sites
3
pi
0.00139
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GACGGGAGAGCAAGTCAAAA GAGGTTAAGCGTACTCTGGC 50.0 55.0 58.1 58.1 1802-1898 bp 1.0 82.0
trnV-GAC-rps12_p2 GACGGGAGAGCAAGTCAAAA GGTTAAGCGTACTCTGGCAA 50.0 50.0 58.1 57.9 1800-1896 bp 1.0 81.9
trnV-GAC-rps12_p3 GATGACTTCCACCACGTCAA GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1739-1835 bp 1.0 81.7
trnV-GAC-rps12_p4 AACTGATGACTTCCACCACG GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1743-1839 bp 1.0 81.7
trnV-GAC-rps12_p5 ATGACTTCCACCACGTCAAG GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1738-1834 bp 1.0 81.7