Hotspot

ndhF-rpl32

SSC · 116201-117145 · 945 bp

Back to Platycarya

Pi 0.0405

MarkerSeek score 79.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 945
variable_sites: 53
pi: 0.0405
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGCCCCACCCCTATTAACAT	GCTACCCAATACCCCTTCCT	50.0	55.0	58.1	58.5	1000-1105 bp	1.0	83.5
ndhF-rpl32_p2	AGAAGCCCCACCCCTATTAA	GCTACCCAATACCCCTTCCT	50.0	55.0	57.7	58.5	1003-1108 bp	1.0	81.8
ndhF-rpl32_p3	AAGCCCCACCCCTATTAACA	GCTACCCAATACCCCTTCCT	50.0	55.0	58.3	58.5	1001-1106 bp	1.0	81.7
ndhF-rpl32_p4	GAAGCCCCACCCCTATTAAC	GCTACCCAATACCCCTTCCT	55.0	55.0	57.6	58.5	1002-1107 bp	1.0	81.3
ndhF-rpl32_p5	AGCCCCACCCCTATTAACAT	TGCTACCCAATACCCCTTCC	50.0	55.0	58.1	58.8	1001-1106 bp	1.0	81.1