Hotspot

petA-psbJ

LSC · 66053-67226 · 1174 bp

Back to Platycarya

Pi 0.0411

MarkerSeek score 88.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1174
variable_sites: 62
pi: 0.0411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 88.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1276-1319 bp	1.0	84.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1259-1302 bp	1.0	83.7
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1273-1316 bp	1.0	83.5
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1271-1314 bp	1.0	82.1
petA-psbJ_p5	TACGTGTCCAAGGCCTTTTG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.4	57.6	1270-1313 bp	1.0	81.7