Hotspot

accD-psaI

LSC · 62108-62875 · 768 bp

Back to Platycarya

Pi 0.0223

MarkerSeek score 67.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 25
pi: 0.0223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGCGTTCTGAGTGAGT	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.4	58.0	918-981 bp	1.0	79.7
accD-psaI_p2	AAAGGCGTTCTGAGTGAGTT	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.4	58.0	917-980 bp	1.0	79.7
accD-psaI_p3	AAAAGGCGTTCTGAGTGAGT	TCGCAATTGCCGGAAATACT	45.0	45.0	57.4	58.3	908-971 bp	1.0	77.7
accD-psaI_p4	AAAGGCGTTCTGAGTGAGTT	TCGCAATTGCCGGAAATACT	45.0	45.0	57.4	58.3	907-970 bp	1.0	77.7
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	GCCGGAAATACTAGGCCTAC	45.0	55.0	57.4	57.5	899-962 bp	1.0	76.1