Hotspot

psaA-ycf3

LSC · 44725-45464 · 740 bp

Back to Platycarya

Pi 0.00369

MarkerSeek score 63.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 4
pi: 0.00369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	859-943 bp	1.0	77.8
psaA-ycf3_p2	TTGAGAAATGACCGGGTCTG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.5	58.0	926-1010 bp	1.0	77.7
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	CAGAGGCTTGGTTCGATCAA	50.0	50.0	58.5	57.9	874-958 bp	1.0	77.3
psaA-ycf3_p4	TTGAGAAATGACCGGGTCTG	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.5	57.9	941-1025 bp	1.0	77.2
psaA-ycf3_p5	TTGAGAAATGACCGGGTCTG	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.5	58.1	928-1012 bp	1.0	76.9