Hotspot

trnD-GUC-trnY-GUA

LSC · 32791-33253 · 463 bp

Back to Platycarya

Pi 0.0214
MarkerSeek score 73.30
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
463
variable_sites
11
pi
0.0214
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA TGGCAATATGTCTACGCTGG 50.0 50.0 58.5 57.8 512-594 bp 1.0 79.9
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA GGCAATATGTCTACGCTGGT 50.0 50.0 58.5 57.8 511-593 bp 1.0 79.9
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.5 57.5 528-610 bp 1.0 77.6
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GCGGACTGTAAATTCGTTGG 50.0 50.0 58.5 57.5 529-611 bp 1.0 77.6
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GGCGGACTGTAAATTCGTTG 50.0 50.0 58.5 57.5 530-612 bp 1.0 77.6