Hotspot

petN-psbM

LSC · 30451-31417 · 967 bp

Back to Platycarya

Pi 0.0076

MarkerSeek score 63.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 11
pi: 0.0076
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1133-1147 bp	1.0	68.7
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1133-1147 bp	1.0	64.5
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.1	57.6	1134-1148 bp	1.0	62.8
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.9	57.6	1125-1139 bp	1.0	62.7
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAGTCAGCCAA	50.0	40.0	58.2	55.8	1083-1097 bp	1.0	62.6