Hotspot

trnS-GCU-trnG-UCC

LSC · 8805-9649 · 845 bp

Back to Platycarya

Pi 0.0211
MarkerSeek score 70.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
845
variable_sites
25
pi
0.0211
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA GGAACGAATCACACTTTTACCA 50.0 40.9 58.0 56.9 908-921 bp 1.0 61.5
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA TGGAACGAATCACACTTTTACC 50.0 40.9 58.0 56.9 909-922 bp 1.0 61.5
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC GGAACGAATCACACTTTTACCA 50.0 40.9 58.0 56.9 907-920 bp 1.0 61.5
trnS-GCU-trnG-UCC_p4 GACAGAAGCGGAAAGAGAGG GGAACGAATCACACTTTTACCA 55.0 40.9 58.0 56.9 969-982 bp 1.0 61.3
trnS-GCU-trnG-UCC_p5 GACAGAAGCGGAAAGAGAGG TGGAACGAATCACACTTTTACC 55.0 40.9 58.0 56.9 970-983 bp 1.0 61.3