Hotspot

trnK-UUU-rps16

LSC · 4225-5262 · 1038 bp

Back to Platycarya

Pi 0.0107
MarkerSeek score 66.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1038
variable_sites
16
pi
0.0107
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCAGTCGCGGTCTTACAAAG TCAAAGAAGGCGGGAGTTTT 50.0 45.0 57.9 57.6 1186-1230 bp 1.0 80.4
trnK-UUU-rps16_p2 TCAGTCGCGGTCTTACAAAG TTCAAAGAAGGCGGGAGTTT 50.0 45.0 57.9 57.6 1187-1231 bp 1.0 80.4
trnK-UUU-rps16_p3 TCAGTCGCGGTCTTACAAAG TTTCAAAGAAGGCGGGAGTT 50.0 45.0 57.9 57.6 1188-1232 bp 1.0 80.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TCAAAGAAGGCGGGAGTTTT 50.0 45.0 58.2 57.6 1099-1143 bp 1.0 79.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTCAAAGAAGGCGGGAGTTT 50.0 45.0 58.2 57.6 1100-1144 bp 1.0 79.8