Hotspot

rps2

LSC · 15519-16229 · 711 bp

Back to Platostoma

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACCGATTCCATAAATCTATCCGA	ACGGTAGAAGAGAAGGTTCCA	39.1	47.6	57.0	58.1	865-868 bp	1.0	40.1
rps2_p2	ACCGATTCCATAAATCTATCCGA	AGAAGAGAAGGTTCCATCGGA	39.1	47.6	57.0	58.2	860-863 bp	1.0	40.1
rps2_p3	ACCGATTCCATAAATCTATCCGA	TGGCCAATCTACGGTAGAAGA	39.1	47.6	57.0	58.3	875-878 bp	1.0	40.1
rps2_p4	ACCGATTCCATAAATCTATCCGA	TGGCCAATCTACGGTAGAAG	39.1	50.0	57.0	56.7	875-878 bp	1.0	40.1
rps2_p5	ACCGATTCCATAAATCTATCCGA	GAAGAGAAGGTTCCATCGGA	39.1	50.0	57.0	56.6	859-862 bp	1.0	40.1