Hotspot

rpoC2

LSC · 16443-20594 · 4152 bp

Back to Platostoma

Pi 0.000963

MarkerSeek score 54.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4152
variable_sites: 4
pi: 0.000963
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CGCCTTTTTGAATCGATTGGA	CAAATGAGTGCGCATTCTCG	42.9	50.0	57.5	58.2	268 bp	1.0	66.9
rpoC2_p2	TCGCCTTTTTGAATCGATTGG	CAAATGAGTGCGCATTCTCG	42.9	50.0	57.5	58.2	269 bp	1.0	66.9
rpoC2_p3	CGTTCTCGCCTTTTTGAATCG	CAAATGAGTGCGCATTCTCG	47.6	50.0	58.5	58.2	274 bp	1.0	66.7
rpoC2_p4	CGCCTTTTTGAATCGATTGGA	GAACGAATGATCCGTCGAGA	42.9	50.0	57.5	57.5	213 bp	1.0	64.7
rpoC2_p5	TCGCCTTTTTGAATCGATTGG	GAACGAATGATCCGTCGAGA	42.9	50.0	57.5	57.5	214 bp	1.0	64.7