Hotspot

clpP

LSC · 69636-71556 · 1921 bp

Back to Platostoma

Pi 0.00156

MarkerSeek score 55.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1921
variable_sites: 3
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGA	47.6	40.0	57.7	55.5	2083-2097 bp	1.0	49.9
clpP_p2	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAA	47.6	38.1	57.7	56.3	2083-2097 bp	1.0	48.2
clpP_p3	GGGCTGGTTTAGATTGATCCT	ACGTTTCCACATCAAAGTGA	47.6	40.0	57.4	55.5	2082-2096 bp	1.0	47.6
clpP_p4	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAAA	47.6	36.4	57.7	57.0	2083-2097 bp	1.0	46.0
clpP_p5	GGGCTGGTTTAGATTGATCCT	ACGTTTCCACATCAAAGTGAA	47.6	38.1	57.4	56.3	2082-2096 bp	1.0	45.9