Hotspot

atpA

LSC · 10139-11662 · 1524 bp

Back to Platostoma

Pi 0.000656

MarkerSeek score 56.80

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	GGTTCAAATCCTATTGGACGC	CGATGAAAGAAATAACTGATTAGTCT	47.6	30.8	57.3	55.7	1763-1764 bp	1.0	40.0
atpA_p2	GGTTCAAATCCTATTGGACGC	CGATGAAAGAAATAACTGATTAGTC	47.6	32.0	57.3	54.4	1763-1764 bp	1.0	40.0
atpA_p3	GGTTCAAATCCTATTGGACGC	CGATGAAAGAAATAACTGATTAGTCTT	47.6	29.6	57.3	56.3	1763-1764 bp	1.0	40.0
atpA_p4	GGTTCAAATCCTATTGGACGC	CGATGAAAGAAATAACTGATTAGT	47.6	29.2	57.3	53.3	1763-1764 bp	1.0	40.0
atpA_p5	GTTCAAATCCTATTGGACGCA	CGATGAAAGAAATAACTGATTAGTCT	42.9	30.8	56.6	55.7	1762-1763 bp	1.0	40.0