Hotspot

rpl20-rps12

LSC · 68670-69394 · 725 bp

Back to Platostoma

Pi 0.00276

MarkerSeek score 60.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 2
pi: 0.00276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGGGCTATATATCCGCGTTT	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.7	57.8	836 bp	1.0	76.3
rpl20-rps12_p2	CGGGCTATATATCCGCGTTT	ACAAGACAGCCCATTCGAAA	50.0	45.0	57.7	57.7	841 bp	1.0	75.7
rpl20-rps12_p3	CGGGCTATATATCCGCGTTT	AGACAGCCCATTCGAAATGT	50.0	45.0	57.7	57.5	838 bp	1.0	73.9
rpl20-rps12_p4	GTTCTACGTTTCCGGGCTAT	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.4	57.8	848 bp	1.0	73.9
rpl20-rps12_p5	CGGGCTATATATCCGCGTTT	AATGTCACGAAATCCCCTGC	50.0	50.0	57.7	58.5	823 bp	1.0	73.6