Hotspot

rps16-trnQ-UUG

LSC · 5936-6855 · 920 bp

Back to Platostoma

Pi 0.00217
MarkerSeek score 60.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
920
variable_sites
2
pi
0.00217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCCACAACGGGTCATATCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1015-1016 bp 1.0 78.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 993-994 bp 1.0 78.4
rps16-trnQ-UUG_p3 TCCACAACGGGTCATATCCT TAGCAATTGAAATGGGGCGT 50.0 45.0 58.1 58.2 1075-1076 bp 1.0 77.9
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAGCAATTGAAATGGGGCGT 45.0 45.0 58.1 58.2 1053-1054 bp 1.0 77.8
rps16-trnQ-UUG_p5 CCACAACGGGTCATATCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 1014-1015 bp 1.0 73.9