Hotspot

trnK-UUU-rps16

LSC · 4263-4831 · 569 bp

Back to Platostoma

Pi 0.00352

MarkerSeek score 55.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 2
pi: 0.00352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCTAGCCGCACTTAAAAGCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.3	58.0	677-678 bp	1.0	78.1
trnK-UUU-rps16_p2	CCGCACTTAAAAGCCGAGTA	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.3	58.0	672-673 bp	1.0	78.0
trnK-UUU-rps16_p3	TCGCGGTCTTCCAAAGTTTA	AAAAGGAGCTCAACCCACAG	45.0	50.0	57.5	58.0	744-745 bp	1.0	76.0
trnK-UUU-rps16_p4	CGCGGTCTTCCAAAGTTTAC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.4	58.0	743-744 bp	1.0	75.5
trnK-UUU-rps16_p5	TCTAGCCGCACTTAAAAGCC	AAAAAGGAGCTCAACCCACA	50.0	45.0	58.3	57.5	678-679 bp	1.0	74.6