Hotspot

ycf1

IRb · 116872-117216 · 345 bp

Back to Platanus

Pi 0

MarkerSeek score 19.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 345
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	ACGAATGGGGTTGGTGTTAC	55.0	50.0	58.0	58.1	906 bp	1.0	80.2
ycf1_p2	CCCGGAACTTCTTCGTAGTG	ACGAATGGGGTTGGTGTTAC	55.0	50.0	58.0	58.1	811 bp	1.0	80.2
ycf1_p3	CCGGAACTTCTTCGTAGTGG	ACGAATGGGGTTGGTGTTAC	55.0	50.0	58.0	58.1	810 bp	1.0	80.2
ycf1_p4	CCGGAACTTCTTCGTAGTGG	CGAATGGGGTTGGTGTTACA	55.0	50.0	58.0	58.1	809 bp	1.0	80.2
ycf1_p5	GAGCTACTGAGGAACAACGG	CGAATGGGGTTGGTGTTACA	55.0	50.0	58.0	58.1	905 bp	1.0	80.1