Hotspot

ndhF

SSC · 117295-119541 · 2247 bp

Back to Platanus

Pi 0.00178

MarkerSeek score 34.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 6
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCTCGGGTTATGGAAGAAGG	CTATGGGAAGTGAGATGGCG	55.0	55.0	58.0	57.8	2993-2996 bp	1.0	78.8
ndhF_p2	GGGACAGCTCATGATGTTCA	CTATGGGAAGTGAGATGGCG	50.0	55.0	57.6	57.8	2934-2937 bp	1.0	75.9
ndhF_p3	AGAAGGAACCGAGAAGGAGG	CTATGGGAAGTGAGATGGCG	55.0	55.0	58.4	57.8	2979-2982 bp	1.0	75.7
ndhF_p4	TACGGGACAGCTCATGATGT	CTATGGGAAGTGAGATGGCG	50.0	55.0	58.5	57.8	2937-2940 bp	1.0	75.1
ndhF_p5	ACCGAGAAGGAGGTATCAGC	CTATGGGAAGTGAGATGGCG	55.0	55.0	58.6	57.8	2972-2975 bp	1.0	74.4