Hotspot

rpl33-rps18

LSC · 75378-75674 · 297 bp

Back to Platanus

Pi 0.00898

MarkerSeek score 45.70

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 297
variable_sites: 4
pi: 0.00898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	CAGAAAGAGTCGCTTGGGTT	55.0	50.0	58.5	58.1	411 bp	1.0	75.6
rpl33-rps18_p2	CGACACAATACGCCTAGTCG	TTCAGAAAGAGTCGCTTGGG	55.0	50.0	58.5	57.8	413 bp	1.0	75.1
rpl33-rps18_p3	CCGGGCGTTTCCAGATATAT	CAGAAAGAGTCGCTTGGGTT	50.0	50.0	57.2	58.1	444 bp	1.0	73.7
rpl33-rps18_p4	CCGGGCGTTTCCAGATATAT	TTCAGAAAGAGTCGCTTGGG	50.0	50.0	57.2	57.8	446 bp	1.0	73.3
rpl33-rps18_p5	CGACACAATACGCCTAGTCG	GAGTCGCTTGGGTTTATCCA	55.0	50.0	58.5	57.6	405 bp	1.0	73.3