Hotspot

rbcL-accD

LSC · 63469-64327 · 859 bp

Back to Platanus

Pi 0.00311

MarkerSeek score 48.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 859
variable_sites: 4
pi: 0.00311
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	TAGCCCACACCTGTGTTCTA	50.0	50.0	58.0	58.0	1014-1015 bp	1.0	80.0
rbcL-accD_p2	CGTGAAGCTTGCAAATGGAG	AGCACCAAGACTGTCCATTG	50.0	50.0	58.0	58.1	1041-1042 bp	1.0	79.4
rbcL-accD_p3	CGTGAAGCTTGCAAATGGAG	GCACCAAGACTGTCCATTGA	50.0	50.0	58.0	58.1	1040-1041 bp	1.0	79.3
rbcL-accD_p4	GCAAATGGAGCCCTGAACTA	TAGCCCACACCTGTGTTCTA	50.0	50.0	57.9	58.0	1004-1005 bp	1.0	79.2
rbcL-accD_p5	GCAAATGGAGCCCTGAACTA	AGCACCAAGACTGTCCATTG	50.0	50.0	57.9	58.1	1031-1032 bp	1.0	78.6