Hotspot

psaA-ycf3

LSC · 46133-46788 · 656 bp

Back to Platanus

Pi 0.00203

MarkerSeek score 47.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 2
pi: 0.00203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGTTGAAGATAACGAGGCG	50.0	50.0	57.9	58.0	766 bp	1.0	79.2
psaA-ycf3_p2	TACTTCTGATTCCGGCGAAC	TGGTTGAAGATAACGAGGCG	50.0	50.0	57.7	58.0	716 bp	1.0	77.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	839 bp	1.0	77.0
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GGTTGAAGATAACGAGGCGA	50.0	50.0	57.9	57.7	765 bp	1.0	77.0
psaA-ycf3_p5	TTGAGAAATGACCCGGTCTG	TGGTTGAAGATAACGAGGCG	50.0	50.0	57.5	58.0	786 bp	1.0	76.4