Hotspot

rpoB-trnC-GCA

LSC · 28882-30102 · 1221 bp

Back to Platanus

Pi 0.000558

MarkerSeek score 48.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 1
pi: 0.000558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCCGTCAAGCCTTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1359-1383 bp	1.0	80.2
rpoB-trnC-GCA_p2	AGTTCTTCCGTCAAGCCTTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1363-1387 bp	1.0	80.1
rpoB-trnC-GCA_p3	GTTCTTCCGTCAAGCCTTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1362-1386 bp	1.0	80.0
rpoB-trnC-GCA_p4	GAGTTCTTCCGTCAAGCCTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1364-1388 bp	1.0	79.6
rpoB-trnC-GCA_p5	CTTCCGTCAAGCCTTGATCA	GGGGAAAAGGGATTTGCAGT	50.0	50.0	57.9	58.4	1346-1370 bp	1.0	78.0