Hotspot

atpH-atpI

LSC · 15501-16660 · 1160 bp

Back to Platanus

Pi 0.0023

MarkerSeek score 47.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 4
pi: 0.0023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCCG	45.0	45.0	58.0	57.9	1247-1248 bp	1.0	78.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1217-1218 bp	1.0	78.5
atpH-atpI_p3	GGCAGAAATCAGTGGGTTCA	TTTTGCAACTTTAGCTGCCG	50.0	45.0	58.1	57.9	1236-1237 bp	1.0	78.5
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCCG	50.0	45.0	57.9	57.9	1303-1304 bp	1.0	78.3
atpH-atpI_p5	GATACCTTCTACGGCTTGGC	TTTTGCAACTTTAGCTGCCG	55.0	45.0	58.1	57.9	1332-1333 bp	1.0	78.1